Mouse anti-Human Ig (Chain kappa), (Light Chain) Antibody

Catalog No. ABIN614821

Quick Overview for Mouse anti-Human Ig (Chain kappa), (Light Chain) Antibody (ABIN614821)

Target: Ig

Clonality: Monoclonal

Application: Flow Cytometry (FACS)

Clone: TB28-2

Isotype: IgG1

Product Details

Binding Specificity: Chain kappa, Light Chain

Reactivity: Human

Host: Mouse

Cross-Reactivity (Details): Species reactivity (tested):Human

Purification: Protein-A affinity chromatography

Immunogen: Human IgG-kappa myeloma protein

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH ~7.4, 15 mM Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Storer the antibody at 2-8 °C for up to one month or (in aliqouts) at -20 °C for longer.

References

Jourdan, Caraux, De Vos, Fiol, Larroque, Cognot, Bret, Duperray, Hose, Klein: "An in vitro model of differentiation of memory B cells into plasmablasts and plasma cells including detailed phenotypic and molecular characterization." in: Blood, Vol. 114, Issue 25, pp. 5173-81, (2009) (PubMed).

Böttcher, Ritgen, Buske, Gesk, Klapper, Hoster, Hiddemann, Unterhalt, Dreyling, Siebert, Kneba, Pott et al.: "Minimal residual disease detection in mantle cell lymphoma: methods and significance of four-color flow cytometry compared to consensus IGH-polymerase chain reaction at initial staging and for ..." in: Haematologica, Vol. 93, Issue 4, pp. 551-9, (2008) (PubMed).

Cassens, Göhde, Kuling, Gröning, Schlenke, Lehman, Traoré, Servais, Henin, Reichelt, Greve: "Simplified volumetric flow cytometry allows feasible and accurate determination of CD4 T lymphocytes in immunodeficient patients worldwide." in: Antiviral therapy, Vol. 9, Issue 3, pp. 395-405, (2004) (PubMed).

Karandikar, Aquino, McKenna, Kroft: "Transient myeloproliferative disorder and acute myeloid leukemia in Down syndrome. An immunophenotypic analysis." in: American journal of clinical pathology, Vol. 116, Issue 2, pp. 204-10, (2001) (PubMed).

Nakamura, Kubagawa, Cooper: "Heterogeneity of immunoglobulin-associated molecules on human B cells identified by monoclonal antibodies." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 18, pp. 8522-6, (1992) (PubMed).

Target Details

Target: Ig

Alternative Name: Immunoglobulin

Background: Immunoglobulin classes share the same basic four polypeptide chain structure of two heavy chains (five heavy chains types) and two light chains (kappa, lambda, both having a molecular weight of 22.5 kDa). Kappa and lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of kappa to lambda is 70:30.